Ottemann Lab Publications

You can see all the Ottemann Lab Publications via Pubmed: Ottemann Lab Publications

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    2019: What it takes to live in a gland

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    Ph.D. student Christina Yang turned her literature review on how to live in a gland into a publication called Control of Bacterial Colonization in the Glands and Crypts (Current Opinion in Microbiology 47:38-44, 2019). We got a share link to the Journal Version, that allows free access until January 17, 2019: https://authors.elsevier.com/a/1Y8HR4tPFpHoev.

    After that day, you can access the author copy here.


  • 2018: Flagella hold it all together

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    Postdoc Skander Hathroubi and undergraduate student Julia Zerebinski report the exciting finding that H. pylori biofilms express large amounts of flagellar apparatus genes, and these structures appear to hold the biofilm together. 

    Helicobacter pylori Biofilm Involves a Multigene Stress-Biased Response, Including a Structural Role for Flagella
    Skander Hathroubi, Julia Zerebinski, Karen M. Ottemann

  • 2018: Running the ROS Gauntlet

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    Ph.D. student Kieran Collins went out with a bang with his finding that ROS chemotaxis is critical for H. pylori to colonize multiple gastric glands.  This work made use mutant mice lacking either epithelial-produced or immune cell produced ROS. In these strains, the defect of TlpD mutants was reversed. The current model is that multiple types of host ROS limit gland colonization and that bacteria have evolved specific mechanisms to migrate through this gauntlet to establish in the glands.

    Collins, K. D., Hu, S., Grasberger, H., Kao, J. Y. & Ottemann, K. M. (2018). Chemotaxis allows bacteria to overcome host-generated reactive oxygen species that constrain gland colonization. Infect Immun 86, e00878–17 (N. E. Freitag, Ed.). http://iai.asm.org/content/86/5/e00878-17.abstract

    Also covered as an article of interest: http://iai.asm.org/content/86/5/e00197-18.full?site=InfectImmun&utm_source=TrendMDInfectImmun&utm_medium=TrendMDInfectImmun&utm_campaign=trendmdalljournals_0


  • 2017: TlpC directly binds the chemoattractant lactate

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    The Ottemann lab collaborated with crystallographer Anna Roujeinikova's lab to identify lacatate as an H. pylori chemoattractant. The Roujeinikova lab crystallized the ligand binding domain of TlpC, and found that it had bound a lactate molecule. Ottemann Lab Ph.D. student Kevin Johnson showed that lactate is an H. pylori chemoattractant that is sensed by TlpC. TlpC is the first chemoreceptor in its family to bind a ligand in the membrane proximal CACHE domain. Lactate is a preferred food for H. pylori, so tracking it down makes sense.  

    Machuca, M. A., Johnson, K. S., Liu, Y. C., Steer, D. L., Ottemann, K. M. & Roujeinikova, A. (2017). Helicobacter pylori chemoreceptor TlpC mediates chemotaxis to lactate. Sci Rep 7, 14089. Nature Publishing Group. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658362/


  • 2017: Full genome sequences of SS1 and PMSS1 reveal intriguing variation

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    Jenny Draper--who completed her Ph.D. in the Ottemann Lab--, Karen Ottemann, Kevin Karplus, Jay Solnick and colleagues publish the H. pylori SS1 and PMSS1 genomes, and find there is intriguing within-genome variation and amplification of the cagA, achieving up to 5 copies. 

    Draper, J. L., Hansen, L. M., Bernick, D. L., Abedrabbo, S., Underwood, J. G., Kong, N., Huang, B. C., Weis, A. M., Weimer, B. C. et al. (2017). Fallacy of the Unique Genome: Sequence Diversity within Single Helicobacter pylori Strains. mBio 8http://mbio.asm.org/content/8/1/e02321-16

    News coverage at: http://www.biotechniques.com/news/Real-vs.-Reference-Genomes/biotechniques-365665.html

    Twitter: https://twitter.com/mbiojournal/status/834538317517639680


  • 2017: Two coupling proteins--CheV1 and CheW--are needed to build polar chemoreceptor arrays

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    Ph.D. student Samar Abedrabbo and M.S. student Juan Castellon made the intriguing discovery that CheV1 works with CheW to build the massive chemoreceptor arrays. 

    Abbedrabbo, S., J. Castellon, K. Johnson, K.D. Collins, and K.M. Ottemann. (2017) Cooperation of two distinct coupling proteins creates chemosensory network connections. Proceedings of the National Academies of Science. 14:2970-2975. http://www.pnas.org/content/114/11/2970.long